Characterization of a protein corona formation
on single EVs using super-resolution imaging

Super-resolution microscopy helps better characterize small structures under 200 nm in size, such as Extracellular Vesicles and their associated proteins. A recent study by Martin Wolf and colleagues from the laboratory of Prof. Dirk Strunk at the Paracelsus Medical University used the Nanoimager to study the functionality of allogeneic placental-expanded stromal cell EVs with or without a protein corona.

Check the application note below to learn how Wolf et al used two-color dSTORM imaging to directly visualize and characterize the protein corona associated with individual EVs. Importantly, EVs depleted of this corona were no longer functional in proangiogenesis and immunomodulation.