Super-resolution microscopy helps better characterize small structures under 200 nm in size, such as Extracellular Vesicles and their associated proteins. A recent study by Martin Wolf and colleagues from the laboratory of Prof. Dirk Strunk at the Paracelsus Medical University used the Nanoimager to study the functionality of allogeneic placental-expanded stromal cell EVs with or without a protein corona.
Check the application note below to learn how Wolf et al used two-color dSTORM imaging to directly visualize and characterize the protein corona associated with individual EVs. Importantly, EVs depleted of this corona were no longer functional in proangiogenesis and immunomodulation.
![App Note image - Characterization of a protein corona formation on single EVs using super-resolution imaging App Note image - Characterization of a protein corona formation on single EVs using super-resolution imaging](https://pages.oni.bio/hs-fs/hubfs/App%20notes/App%20Note%20image%20-%20Characterization%20of%20a%20protein%20corona%20formation%20on%20single%20EVs%20using%20super-resolution%20imaging.jpg?width=791&height=1028&name=App%20Note%20image%20-%20Characterization%20of%20a%20protein%20corona%20formation%20on%20single%20EVs%20using%20super-resolution%20imaging.jpg)