In this app note, we discuss how super-resolution microscopy can be used to reveal changes to nanoscale receptor recruitment in neural synapses.
The Nanoimager is capable of performing multicolor super-resolution imaging in neuronal cultures. Among these techniques, dSTORM is commonly used to determine the molecular organization of protein complexes in synapses. With the Nanoimager it is possible to:
- Visualize synaptic protein organization with 20 nm resolution.
- Perform multicolor imaging of synaptic protein complexes, providing co-localization information of different targets at the nanometer scale.
- Determine quantitative subsynaptic parameters, such as the area, density and number of protein clusters per synapse or relative protein number per cluster